Journal: Scientific reports

Article Title: Thioredoxin-1 inhibits NLRP3-mediated pyroptosis by regulating TXNIP in models of Alzheimer's disease.

doi: 10.1038/s41598-025-01636-5

Figure Lengend Snippet: Fig. 3. Trx-1 overexpression inhibited the activation of NLRP3 in the hippocampus of APP/PS1 mice. (A) The WB detection of NLRP3, caspase-1 and IL-1β in the hippocampus of APP/PS1 mice. (B) Gray scale analysis of NLRP3. (C) Gray scale analysis of caspase-1. (D) Gray scale analysis of IL-1β. Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD from three independent experiments (n = 5 in WT + AAV-Trx-1 and WT + AAV-Vector groups, n = 4 in APP/PS1 + AAV- Vector and APP/PS1 + AAV-Trx-1 groups). **P < 0.01, ***P < 0.001.

Article Snippet: Primary Aβ (TA500973S), caspase-1 (TA383869) and β-actin antibody (TA811000) were purchased from Origene (Rockville, USA).

Techniques: Over Expression, Activation Assay, Plasmid Preparation

Journal: Scientific reports

Article Title: Thioredoxin-1 inhibits NLRP3-mediated pyroptosis by regulating TXNIP in models of Alzheimer's disease.

doi: 10.1038/s41598-025-01636-5

Figure Lengend Snippet: Fig. 5. Trx-1 overexpression in HT22 cells resisted the neurotoxicity of Aβ25−35. (A) Cell morphology of HT22 cells overexpressing human Trx-1 treated for 48 h with Aβ25−35 (40 µM). (B) Cell viability was measured by MTT analysis. Trx-1 overexpression reversed the viability of HT22 cells. (C) The increase in the extracellular LDH content was detected with LDH Cytotoxicity Assay Kit. Trx-1 overexpression inhibited the release of LDH. (D) Overexpression of Trx-1 inhibited Aβ25−35-induced apoptosis in HT22 cells (red fluorescence). Annexin V-mCherry can be coupled to phosphatidylserine on the outer membrane of apoptotic cells, and mCherry labeled with red fluorescent protein can indicate apoptotic cells. (E) Statistical analysis of Aβ25−35-induced apoptosis in HT22 cells. Red fluorescence intensity was quantified by using ImageJ. (F) Overexpression of Trx-1 inhibited the Aβ25−35-induced caspase-3 activation in HT22 cells. (G) Gray scale analysis of caspase-3 expression in HT22 cells; The quantitative analysis was based on three independent experiments. Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Primary Aβ (TA500973S), caspase-1 (TA383869) and β-actin antibody (TA811000) were purchased from Origene (Rockville, USA).

Techniques: Over Expression, LDH Cytotoxicity Assay, Fluorescence, Membrane, Labeling, Activation Assay, Expressing

Journal: Scientific reports

Article Title: Thioredoxin-1 inhibits NLRP3-mediated pyroptosis by regulating TXNIP in models of Alzheimer's disease.

doi: 10.1038/s41598-025-01636-5

Figure Lengend Snippet: Fig. 6. Trx-1 inhibited the activation of NLRP3 by Aβ25−35 in HT22 cells. (A) The WB detection of NLRP3, caspase-1, GSDMD and IL-1β in WT HT22 and Trx-1 overexpressing HT22 cells treated by Aβ25−35 (40 µM). (B) Gray scale analysis of NLRP3. (C) Gray scale analysis of caspase-1. (D) Gray scale analysis of GSDMD. (E) Gray scale analysis of IL-1β. (F) Immunofluorescence staining of NLRP3 in WT HT22 and Trx-1 overexpressing HT22 cells treated by Aβ25−35 (40 µM). (G) Transfection with Trx-1 siRNA for 24 h downregulated the protein levels of Trx-1 in HT22 cells. (H) Trx-1 knockdown in HT22 cells by siRNA transfection further aggravated the activation of NLRP3. (I) The increase of NLRP3 by Aβ25−35 was abolished in SH-SY5Y cells treated with rhTrx-1 (50 µg/ml). Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD from three independent experiments. The quantitative analysis was based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Primary Aβ (TA500973S), caspase-1 (TA383869) and β-actin antibody (TA811000) were purchased from Origene (Rockville, USA).

Techniques: Activation Assay, Immunofluorescence, Staining, Transfection, Knockdown

Journal: International Journal of Molecular Sciences

Article Title: Melatonin Inhibits Hypoxia-Induced Alzheimer’s Disease Pathogenesis by Regulating the Amyloidogenic Pathway in Human Neuroblastoma Cells

doi: 10.3390/ijms25105225

Figure Lengend Snippet: The list of antibodies.

Article Snippet: Mouse anti-APP-C99 , MABN380 , 1:2000 , Merck Millipore, Temecula, CA, USA.

Techniques: